The Coming Alzheimer’s Epidemic, the Cholinergic Hypothesis, and Nerve Growth Factor Gene Therapy

07Oct14

Alzheimer’s disease (AD) is by far the number one cause of dementia, and the number one risk factor for AD is age–with almost 50% of people above the age of 85 suffering from the disease.

Common Dementia SyndromesAlzheimer Disease Prevalence and Projected Population

With the aging baby boomer generation and medical advances squaring off the life expectancy curve, the United States is projected to have over 9 million cases of AD by 2050. This huge expected increase in cases has been dubbed by some as the “Alzheimer’s Disease Epidemic.”

Alzheimer’s disease is marked primarily by memory loss and inability to form new memories, but it can also impair language, higher thinking, visuospatial skills, and can even cause personality changes and delusions. By erasing patients’ identities, memories, and personalities, Alzheimer’s disease robs patients of their humanity, and can be devastating for the friends and family watching the disease unfold.

Auguste D - First Patient with Alzheimer'sAuguste D.’s story

Alzheimer’s disease was first described in 1901, a patient Auguste D., a 51-year-old woman, suffering from debilitating memory loss, deficits, and precatory delusions. She underwent a progressive decline and died five years later. The case was described by Alois Alzheimer a German psychiatrist and neuropathologist (though now the disease is treated by neurologists).

The fact that Alzheimer was a pathologist was of crucial importance. Because of it, he performed an autopsy on Auguste D. and discovered proteinaceous plaques, and neurofibrillary tangles in the woman’s brain, which to this day remain the pathological hallmarks of Alzheimer’s disease:Alzheimer's Disease Brain - Senile Plaques Neurofibrillary Tangles - Pathalogical Hallmarks

Alzheimer’s mentor, Kraepelin, was the first to use the eponym “Alzheimer’s Disease” in 1904, and his writing about the disease was prescient:“Although the anatomical findings suggest that we are dealing with a particularly serious form of senile dementia… this disease sometimes starts as early as in the late forties.”

This clinical observation was borne out by later genetics. There is an early onset familial variant of the AD, that is caused by rare mutations. Familial AD is devastating, otherwise normal, healthy patients in their 40s or younger, but luckily it accounts for a minority of cases. The later-onset variant has complex genetic and environmental risk factors and accounts for the vast majority of cases. In this variant genetic factors such as the ApoE4 allele are known to increase the risk of Alzheimer’s, but a doctor cannot genetically predict who will get it and who won’t.

Genetics of Alzheimer's Disease - Familial Early Onset and Sporadic Late Onset

What was really interesting about the first three genes identified in familial Alzheimer’s disease is that they are part of the same pathway. These genes code for the amyloid precursor protein (APP), and presenilin 1 and 2. Amyloid precursor protein is cleaved by the “gamma secretase complex,” an enzyme that is made up of multiple subunits, including presinilin 1 & 2. If APP is cleaved at the wrong locations, it forms a protein fragment beta-amyloid, which can induce other proteins to misfold, forming a protein aggregate that grows like a snowball rolling down a hill. This protein aggregate eventually forms a giant extracellular plaque—the very same plaques that Alzheimer’s described in his patient Auguste D!

APP Beta-Amyloid Schematic Enzymes Cleavage Presinillin 1 & 2 APP Beta-Amyloid Schematic Plaque Formation Aggregate

The Amyloid Cascade Hypothesis

This genetic work led to the amyloid cascade hypothesis: that extracellular amyloid accumulates and initiates a sequence of events, eventually leading to neurotoxicity and clinical symptoms in AD. This hypothesis has inspired a number of clinical trials to test drugs to treat or slow the course of Alzheimer’s, by attempting to stop the formation or remove these beta-amyloid plaques. Even when trials have been successful y removed patient’s plaques, patients have not shown clinical improvements. It is unclear whether the amyloid cascade hypothesis is wrong (and perhaps amyloid plaques correlate with but do not cause AD), or whether we are just starting our treatments too late once the cascade of neurodegeneration is initiated–but that these treatments could be successful if we used them on patients earlier in their disease course.

In fact we know that patients with Alzheimer’s disease don’t show clinical symptoms until late in the disease, perhaps due to a phenomenon known as cognitive reserve. New techniques however, allow us to determine whether people showing subtle cognitive deficits are at high or low risk of developing Alzhiemer’s, and therefore we can continue to test the amyloid cascade hypothesis by perforing clinical trials using the drugs we know clear plaques on people who are of high risk of developing Alzheimer’s.

For example, the same proteins found in the plaques and tangles in Alzheimer’s disease brains such as amyloid beta make their way into the cerebrospinal fluid which coats the brain and spinal cord. In patients with mild cognitive impairment–meaning they are starting to show cognitive problems compared to age-matched, education-matched controls, but not severe enough to be classified as Alzheimer’s–we can perform lumbar punctures to harvest their cerebrospinal fluid. Then by measuring levels of these proteins found in plaques, we can predict which of these patients with MCI are at high risk of progressing to Alzheimer’s.

Progession to AD Normal CSF Pathological CSF Hansson et alHansson et al., Lancet Neurol (2006)

Additionally, we can look at the burden and distribution of plaques in the brain using radioactive PET ligands that bind to the plaques:

awn320 1310..1323

And we are developing techniques to non-invasively visualize and quantify the burden of this plaques in retina: Amyloid Plaques in Retina

The Cholinergic Hypothesis

While the previously-described techniques will inevitably assist with the diagnosing AD, the amyloid cascade hypothesis is still unproven, and so far has not lead to any effective therapy. Currently, one of the best treatments for Alzheimer’s disease is lifestyle modification: labeling and arranging one’s life as memory declines. The mainstay of pharmacological approach for AD are cholinesterase inhibitors developed in the 1990s and 2000s, which can improve performance on cognitive tests.

Acetycholinesterase mechanism Ach

These drugs block acetylcholinesterase, blocking the breakdown of the neurotransmitter acetylcholine into acetate and choline, so the drugs therefore they increase levels of acetylcholine in the synapse and signaling to the postsynaptic cell.

You might be wondering, how do these drugs help treat Alzheimer’s if it is a disease caused by plaques, tangles and neurodegeneration? Why did we develop and test them in the first place?

These drugs were actually developed based on an earlier theory of Alzheimer’s the cholinergic hypothesis.

In the 1960s and ‘70s psychologists gave young healthy subjects cholinergic inhibitors (which prevent acetylcholine from signaling to post-synaptic cells). These young healthy subjects had memory problems and cognitive problems that resembled patients with Alzheimer’s disease, showing that acetylcholine was important for memory.

Cholinergic projections in brain basal forebrain nucleus of Meynert

Most of the brain’s supply of acetylcholine derives from the nucleus basalis of Meynert in the basal forebrain, so doctors investigated this area during the autopsies of patients with Alzheimer’s and showed that the nucleus basalis seems to be particularly vulnerable to the neurodegeneration seen in AD. Patients showed decreased numbers of healthy neurons in this area, and decreased cholinergic projections to the hippocampus and entorhinal cortex (areas we also knew were important for memory), suggesting that patients with AD may have decreased acetylcholine.

Going back to the drugs we use for Alzheimer’s cholinesterase inhibitors, these drugs are thought to boost the remaining acetylcholine signaling going on in synapses, similar to how SSRIs increase the amount of serotonin. However, because they are not preventing the neurodegeneration that is causing AD, disease progression continues and causes massive neurodegeneration, eventually killing patients by interfering with their ability to swallow and breath.

Hypothesized Treatment effect of cholinesterase inhibitors in AD

However, if the cholinergic hypothesis is correct, and disruptions to the acetylcholine signaling from the basal forebrain is especially important for the symptoms of AD, then maybe if we can selectively prevent degeneration of this area we can slow the disease. Currently clinical trials are delivering of Nerve Growth Factor (NGF) to the basal forebrain using gene therapy approaches—implanting stem cells that produce NGF or using viruses to modify cells in the basal forebrain to produce it themselves. Nerve Growth factor is known to increase the growth and vitality of cholinergic neurons, and may therefore help preserve the cells as they suffer early insults of Alzheimer’s.

Even if Nerve Growth Factor gene therapy can just slow the disease, we can give patients more time with their memories intact to enjoy their families and a dignified independent life. We can decrease the time families and friends spend caring for a person who’s face the recognize, but whose mind becomes increasingly unfamiliar, and we can decrease the time patients have to spend institutionalized or with a full time caretaker.

Clinical research is slow, and research is never certain, but the new approaches to Alzheimer’s give hope to a Alzheimer’s disease—a disease where prognoses for patients like Auguste D. have remained gloomy for over a century, and where an epidemic of new cases looms ahead in the near future.

Advertisements


No Responses Yet to “The Coming Alzheimer’s Epidemic, the Cholinergic Hypothesis, and Nerve Growth Factor Gene Therapy”

  1. Leave a Comment

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s


%d bloggers like this: